Quantitation of proteolytic enzymes using N-succinyl-L-Ala-L-Ala-L-Pro-L-Phe-p-nitroanilide has been adapted to flow-injection analysis. This procedure has been developed using two different proteases: subtilisin and chymotrypsin. For both enzymes the influence of substrate concentration on spectrop
Determination of subtilisin proteolytic activity by flow-injection analysis with fluorescence detection
β Scribed by C.Hayes Powell; R.Brett Nelson
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 379 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0731-7085
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
A flow injection analysis method for determining L-carnitine is reported. The system uses the enzyme L-carnitine dehydrogenase covalently immobilized to Eupergit C. The NADH produced by the action of the enzyme, which is proportional to the L-carnitine concentration, is quantified using fluorescence
An novel analytical method consisting of flow-injection sampling and chemiluminescence (CL) detection for determination of anticancer drug hydroxycamptothecinum (HCPT) is described. It is based on the CL reaction of potassium permanganate-formaldehyde-HCPT system. The formaldehyde-sensitized CL emis
Flow injection analysis (F1A)/chemiluminescence detection has been used to the determination of Al(III), Zn(II), Cd(II), and In(III). Fluorescent compound which was formed by complexation of a metal ion and 8-hydroxyquinoline was excited by the chemiluminescence reaction of bis(2,4,6trichlorophenyl)