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Determination of single-strand breaks in DNA using neutral sucrose gradients

✍ Scribed by P. Crine; W.G. Verly

Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
779 KB
Volume
75
Category
Article
ISSN
0003-2697

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✦ Synopsis

Separate estimations of intact apurinic sites and single-strand breaks in DNA necessitates the use of neutral sucrose gradients for sedimentation analysis after denaturation with formamide or with NaOH followed by reneutralization. The number of breaks per strand in the denatured sample, relative to a control, can be determined with the computer program of Gillespie et al. 6; the particular equation for denatured DNA in neutral sucrose gradient that relates the molecular weight and the sedimentation rate is given. The reliability of the whole technique was proven in an experiment with T7 phage [3ZP]DNA in which the 32P transmutations into ?S were the origin of the strand breaks. 583

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