Determination of rhodanese in plants
β Scribed by Reinhard Lieberei; Dirk Selmar
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 360 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0031-9422
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A colorimetric method for the assay of rhodanese activity based on the continuous determination of the sulfite product is described. 5-Ethylphenazinium ethyl sulfate is used as the intermediate electron carrier between sulfite and nitroblue tetrazolium to produce the colored reduced species. The pre
The biosynthesis of rhodanese was studied in human hepatoma cell lines by immunoblotting and pulse-labeling experiments using polyclonal antibodies raised against the bovine liver enzyme. Rhodanese, partially purified from human liver, showed an apparent molecular weight of 33,000 daltons, coinciden