Determination of prolidase activity using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
β Scribed by Biji T Kurien; Nisha C Patel; Andrew C Porter; Sadamu Kurono; Hiroyuki Matsumoto; Heng Wang; R Hal Scofield
- Book ID
- 108047711
- Publisher
- Elsevier Science
- Year
- 2004
- Tongue
- English
- Weight
- 378 KB
- Volume
- 331
- Category
- Article
- ISSN
- 0003-2697
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The ionization of nucleosides in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was systematically investigated using adenine (A), thymine (T), guanine (G) and cytosine (C) with several common matrices. Experimental results of the protonation and deprotonation of the ba
## Ribonuclease (RNase) B incubated with purified enzymes, whole bacterial cultures, or their separated components-cells and supernates-have been directly analyzed by matrix-assisted laser desorption/ ionization-time of flight mass spectrometry (MALDI-ToF) to detect exomannosidases and to evaluate