𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Determination of polyamines in urine of normal human and cancer patients by capillary gas chromatography

✍ Scribed by Xiaochun Jiang

Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
414 KB
Volume
4
Category
Article
ISSN
0269-3879

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

A capillary gas chromatographic method is described for the determination of polyamines (putrescine, spermidine and spermine) in the urine of normal human and cancer patients. Morning urine after acid hydrolysis is cleaned up on a silica gel column and derivatized with trifluoroacetic‐anhydride. Creatinine in human urine is used as internal standard. Recoveries of polyamines are 96.7% putrescine, 102.6% spermidine (Spd), and 98.7% spermine. SD of the method for Spd is 1.949 ± 0.041 (μg/mg creatinine, mean ±SD, n = 5). The results show that the mean level of polyamines in cancer patients urine is much higher than that in normal human urine. The mean of total polyamines in the normal human and the cancer patients is 2.01 and 44.74, respectively (g/mg creatinine).

📜 SIMILAR VOLUMES

Determination of amino acid enantiomers
Determination of amino acid enantiomers in human urine and blood serum by gas chromatography–mass spectrometry
✍ Hans Brückner; Andreas Schieber 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 103 KB 👁 2 views

## Abstract Amino acid (AA) enantiomers were determined as __N(O)__‐pentafluoropropionyl‐(2)‐propyl esters by chiral gas chromatography–mass spectrometry (GC‐MS) in 24 h samples of the urine of three healthy volunteers and in their blood sera. In urine the largest amounts were determined for D‐Ser

Determination of the enantiomeric compos
Determination of the enantiomeric composition of mexiletine and its four hydroxylated metabolites in urine by enantioselective capillary gas chromatography
✍ Beate Knoche; Bärbel Gehrcke; Wilfried A. König; Irving W. Wainer 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 495 KB 👁 2 views

The enantiomers of mexiletine and four of its hydroxylated metabolites were directly separated by capillary gas chromatography using a heptakis(6-0-tert-butyldimethylsilyl-2,3-di-O-methyl)-p-cyclodextrin column. The method was applied to the analysis of urine samples from cancer patients who were tr