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Determination of plastoquinone-9 in chloroplasts by high pressure liquid chromatography

โœ Scribed by A. Lominski; K.G


Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
411 KB
Volume
20
Category
Article
ISSN
0031-9422

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A rapid, precise, and simple HPLC method provides an assay of hydroxyproline from tissue extracts or solutions of collagen. Samples are hydrolyzed with 6 N HCl, derivatized with phenyl isothiocyanate, and chromatographed on a small, C18 reverse-phase HPLC column. Hydroxyproline (Hyp) is separated fr