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Determination of plasma heparin by polybrene neutralization

โœ Scribed by Patricia V. Nash; Thorir D. Bjornsson

Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
432 KB
Volume
138
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A method for determining heparin activity (in unit/ml) in human plasma is described. The method is based on neutralization of heparin by Polybrene, a polymerized quaternary ammonium salt. It uses serial incubations of plasma with increasing amounts of Polybmne in conjunction with thrombin-induced coagulation times to &fine the amount of Polybrene required to neutral& heparin in the sample. Reference curves involve linear relationships between amounts of Polybrene required to neutralize known amounts of heparin. Coefficients of variation for the assay vary from 4 to 10% over the range of 0.05 to 1.0 unit/ml.

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โœ P. Oreste; M. Peruzzi; P. Stella ๐Ÿ“‚ Article ๐Ÿ“… 1993 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 445 KB

A sensitive method has been developed for extracting and analyzing heparin from plasma after intravenous and subcutaneous administration in humans and rabbits. The glycosaminoglycans are precipitated from the biological fluid as cetylpyridinium salt, and heparin is cleaved with heparinase. The react