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Determination of plasma and serum homocysteine by high-performance liquid chromatography with fluorescence detection

✍ Scribed by G Minniti; A Piana; U Armani; R Cerone

Book ID
108341620
Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
71 KB
Volume
828
Category
Article
ISSN
1873-3778

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Determination of homocysteine in plasma
Determination of homocysteine in plasma by liquid chromatography with fluorescence detection
✍ FranΓ§ois C. Gautier; Christophe Berneron; Philippe J. Douce πŸ“‚ Article πŸ“… 1999 πŸ› John Wiley and Sons 🌐 English βš– 54 KB πŸ‘ 2 views

A liquid chromatographic (LC) method to assay homocysteine in plasma at micro-molar levels is described. The procedure consists of protein precipitation with trichloroacetic acid and a centrifugation step. The supernatant is then derivatized with a thiol-specific fluorochromophore and LC assayed wit