Determination of phenoxymethyl penicilloic acid and phenoxyethyl penicilloic acid in urine in the presence of the parent penicillins

✍ Scribed by Jan Birner

• Book ID: 102914510
• Publisher: John Wiley and Sons
• Year: 1970
• Tongue: English
• Weight: 530 KB
• Volume: 59
• Category: Article
• ISSN: 0022-3549
• DOI: 10.1002/jps.2600590606

✦ Synopsis

I I ] Penicillin and the corresponding penicilloic acid are extracted by chloroform from an acidified sample of urine which has been partly saturated by ammonium sulfate. A portion of the extract is dried and redissolved in a small volume of acetone. A measured aliquot is chromatographed on a silica gel thin-layer plate by acetone-acetic acid (19: 1). The separated penicilloic acid is detected by starch-iodine spraying. The zones are transferred into glass tubes, nitrated, and neutralized by ammonia. The absorbance of the yellow supernatant, which is proportional to the concentration of penicilloic acid, is measured spectrophotometrically. Keyphrases 0 Penicillin, penicilloic acid in urine-penicilloic acid determination 0 Phenoxymethyl penicilloic acid-determination in urine Phenoxyethyl penicilloic acid-determination in urine TLC-analysis 0 Starch-iodine spray-TLC spot identification 0 Colorimetric analysis-spectrophotometer Due to their instability in solution, penicillins taken orally or administered parenterally undergo chemical changes, and the breakdown products are excreted from the body in the urine.