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Determination of p-hydroxyphenoxy methyl penicillin in phenoxymethyl penicillin fermentation samples

✍ Scribed by Jan Birner

Publisher: John Wiley and Sons
Year: 1968
Tongue: English
Weight: 213 KB
Volume: 57
Category: Article
ISSN: 0022-3549
DOI: 10.1002/jps.2600570929

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✦ Synopsis

A method based o n paper chromatography and biological assay has been developed for the determination of p-hydroxyphenoxy methyl penicillin in phenoxy methyl penicillin (penicillin V) fermentation samples. The penicillin broth is extracted into methyl isobutyl ketone. The extract is chromatographed in tri licate using Whatman No. 1 paper treated with sodium citrate at pH 5.5 and ethyl eger saturated with 28 percent ammonium sulfate. The penicillins are located by reference t? a strip cut from the chromatogram and developed colorimetrically. The appropriate

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