Determination of organic and inorganic mercury compounds by reverse-phase high-performance liquid chromatography after extraction of the compounds as their dithizonates

✍ Scribed by Wenche Langseth

• Publisher: Elsevier Science
• Year: 1986
• Tongue: English
• Weight: 695 KB
• Volume: 185
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(86)80052-6

✦ Synopsis

A sensitive and selective method is described for the simultaneous determination of morganic and organic mercury compounds. The mercury compounds are extracted into toluene or chloroform with dithizone, and the dithizonates are separated by liquid chromatography on an ODS column. Complete resolution was obtained between methyl-, ethyl-, phenyl-and inorganic mercury with a mobile phase of THF/methanol (2:l) with 0.05 M acetate buffer pH 4 (62 + 38), containing 50 PM EDTA. The mercury chelates were detected spectrophotometrically at 475 nm. The detection limits were at the subnanogram level. The method is applicable to human urine, tap water and tomatoes.