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A reliable quantitative method for the analysis of phenolic compounds in Brazilian propolis by reverse phase high performance liquid chromatography

✍ Scribed by João P. B. de Sousa; Paula C. P. Bueno; Luiz E. Gregório; Ademar A. da Silva Filho; Niege A. J. C. Furtado; Márcia L. de Sousa; Jairo K. Bastos

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
877 KB
Volume
30
Category
Article
ISSN
1615-9306

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✦ Synopsis

Abstract

A sensitive and reliable RP‐HPLC method was developed using a C18 CLC‐ODS (M) – 4.6×250 mm^2^ column and gradient elution for the analysis of phenolic compounds in propolis raw material and its products. A procedure for the extraction of phenolic compounds using aqueous ethanol (90%) with the addition of veratraldehyde as the internal standard (IS) was developed allowing to quantify ten compounds: caffeic acid, coumaric acid, ferulic acid, cinnamic acid, aromadendrin‐4′‐methyl ether (AME), isosakuranetin, drupanin, artepellin C, baccharin, and 2,2‐dimethyl‐6‐carboxyethenyl‐2__H__‐1‐benzopyran acid (DCBEN). The developed method gave good detection response and linearity in the range of 20.83–533.33 μg/mL.

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