The glycosylation of pharmaglycoproteins from recombinant cell lines can be affected by an uncontrolled accumulation of ammonium in the medium. Glucosamine-6-phosphate isomerase (GPI) has been proposed as the key enzyme responsible for elevating the intracellular UDP-N-acetylhexosamine pool (UDPGNAc
Determination of N-acetylhexosamines in the presence of -SH compounds
β Scribed by K. Zwierz; J. Bialobrzeska; S. Kreczko
- Publisher
- Elsevier Science
- Year
- 1977
- Tongue
- English
- Weight
- 133 KB
- Volume
- 77
- Category
- Article
- ISSN
- 0003-2697
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A spectrophotometric method for inorganic phosphate determination in the presence of thiol compounds is described. Thiol compounds, which interfere with the measurement of inorganic phosphate by a modification of the method of Gomori, are removed by carboxymethylation by iodoacetate prior to the for
A sensitive colorimetric assay for detecting mucins and glycoproteins rich in O-glycosidically linked saccharides is reported. The method combines the susceptibility of N-acetylgalactosamine terminating O-glycosidically linked saccharides to beta-elimination with the Morgan-Elson reaction for N-acet
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The Lowry procedure has been modified for use in the presence of sulfhydryl compounds or other reducing agents by the addition of chloramine T under acid conditions before adding the alkaline-copper reagent. This method is applicable to the determination of 25-100 pg of protein, and a linear standar