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Determination of medroxyprogesterone acetate residues by CE immunoassay with chemiluminescence detection

✍ Scribed by Chi-Fang Peng; Tie-Ming Huo; Li-Qiang Liu; Xiao-Gang Chu; Chuan-Lai Xu

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
264 KB
Volume
28
Category
Article
ISSN
0173-0835

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✦ Synopsis

Abstract

A rapid and simple method is developed for the determination of medroxyprogesterone acetate (MPA) by CE immunoassay with chemiluminescence (CL). This method is based on the competitive reactions between horseradish peroxidase (HRP)‐labeled MPA (MPA‐HRP) and free MPA with anti‐MPA antiserum. The influencing factors on the electrophoresis and CL detection were studied completely and the optimal conditions of separation and determination were obtained. The linear range was 2.0–50 nmol/L and the LOD for MPA was 0.9 nmol/L. The present method was applied to the analysis of pork tissues.

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