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Determination of Manganese Peroxidase Activity with 3-Methyl-2-benzothiazolinone Hydrazone and 3-(Dimethylamino)benzoic Acid

✍ Scribed by M.D. Castillo; J. Stenstrom; P. Ander

Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
479 KB
Volume
218
Category
Article
ISSN
0003-2697

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✦ Synopsis

This method was proposed earlier for measuring glucose in a peroxidase-glucose oxidase system but has not been studied for determination of manganese peroxidase (MnP) activity. The assay is based on the oxidative coupling of 3-methyl-2-benzothiazolinone hydrazone (MBTH) and 3-(dimethylamino)benzoic acid (DMAB). The reaction of MBTH and DMAB in the presence of H2O2, Mn2+, and MnP gives a deep purple-blue color with a broad absorption band with a peak at 590 nm. The extinction coefficient is high (53,000 M-1 cm-1), so low MnP activities can be detected. Lignin peroxidase and laccase, usually present in cultures of white rot fungi, gave little or no interference at the concentrations tested. However, slight interference from very high LiP activity may occur at very low MnP activity.

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