✦ LIBER ✦
A new peroxidase color reaction: Oxidative coupling of 3-methyl-2-benzothiazolinone hydrazone (MBTH) with its formaldehyde azine. Application to glucose and choline oxidases
✍ Scribed by Dante J. Capaldi; Keith E. Taylor
- Publisher
- Elsevier Science
- Year
- 1983
- Tongue
- English
- Weight
- 538 KB
- Volume
- 129
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
Hydrogen peroxide in the presence of horseradish peroxidase effects the oxidative coupling of 3-methyl-2-benzothiazolinone hydrazone with its formaldehyde azine to form a tetraazapentamethine dye. The blue chromophore, when formed at pH 3.5 and quenched with acetone or 1 N hydrochloric acid, has an extinction coefficient of 69 +/- 2 or 55 +/- 2 mM-1 cm-1, respectively. This chromogen system has been adapted for enzymatic determinations of hydrogen peroxide and of glucose in the 10- to 45-nmol range and of choline in the 5- to 20-nmol range.