Disc electrophoresis has been widely used as .a procedure for separating proteins. In many instances the gels have been analyzed for radioactivity and for protein content. However, the only available method for determining protein content has been microdensitometry, which is complex and requires exp
Determination of malate synthase activity in polyacrylamide gels
β Scribed by Marva J. Volk; Richard N. Trelease; Henry C. Reeves
- Publisher
- Elsevier Science
- Year
- 1974
- Tongue
- English
- Weight
- 585 KB
- Volume
- 58
- Category
- Article
- ISSN
- 0003-2697
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A method for the localization of pyruvate decarboxylase bands in polyacrylamide gels after electrophoresis using 1,2-dianilinoethane in dilute acetic acid as reagent for acetaldehyde formed from pyruvate by the holoenzyme is described. A white condensation product forms in the bands and precipitates
Swelling behavior of polyacrylamide (PAAm) and polyacrylamide-co-polyacrylic acid (PAAm-co-PAAc) gels was investigated in aqueous solutions of monodisperse PAAms with molecular weights ( M V w ) ranging from 1.5 1 10 3 to 5 1 10 6 g/mol. The volume of the gels decreases as the PAAm concentration in