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Determination of major carotenoids in vegetables by capillary electrochromatography

✍ Scribed by José M. Herrero-Martínez; Sebastiaan Eeltink; Peter J. Schoenmakers; Wim Th. Kok; Guillermo Ramis-Ramos

Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
982 KB
Volume
29
Category
Article
ISSN
1615-9306

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✦ Synopsis

Determination of major carotenoids in vegetables by capillary electrochromatography

A simple and rapid method for the isocratic separation and determination of carotenoids (carotenes and xanthophylls) in vegetables by CEC is described. The capillary column (100 lm ID, 25 cm effective length) was packed with 3 lm Hypersil ODS particles. The optimized mobile phase contained 60% ACN, 35% THF and 5% of a 5 mM Tris aqueous buffer of pH 8. b-Carotene, lycopene and lutein were separated with efficiencies of 66 000-128 000 plates/m within a short time (less than 12 min for the last peak eluted, 13/139-cis-b-carotene). An excellent resolution of the three carotenoids, as well as partial resolution of their geometrical isomers, was achieved. Application to the determination of the analytes in carrot, tomato, spinach and corn was demonstrated.

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