Determination of Lovastatin (mevinolin) and mevinolinic acid in fermentation liquids
✍ Scribed by Roman Kysilka; Vladimír Křen
- Book ID
- 103149988
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 53 KB
- Volume
- 630
- Category
- Article
- ISSN
- 1873-3778
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Mevinolinic acid (MVA), the major active metabolite of Lovastatin in human blood, is analysed by microbore high-performance liquid chromatography coupled to electrospray tandem mass spectrometry. Quantification is carried out by using methylmevinolinic acid (MMVA) as internal standard. Positive-and
A sensitive and speciÐc method was developed and validated to quantitate lovastatin and its hydroxy acid in mouse and rat plasma. This method employs a solid-phase extraction procedure to isolate lovastatin and its hydroxy acid metabolite from the biological matrices (0.1 ml of mouse or rat plasma).
The production of organic acids and volatile #avor components was measured during ke"r starter culture fermentation. Samples were collected at 0, 5, 10, 15, and 22 h of fermentation ("nal pH"4.6). Samples were analyzed for orotic, citric, pyruvic, uric, lactic, acetic, butyric, propionic and hippuri