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Determination of l-asparagine in biological samples in the presence of l-asparaginase

โœ Scribed by Donatella Gentili; Massimo Zucchetti; Valentino Conter; Giuseppe Masera; Maurizio D'Incalci

Publisher
Elsevier Science
Year
1994
Weight
329 KB
Volume
657
Category
Article
ISSN
0378-4347

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โœ M.J. Almendral; M.J. Porras; A. Alonso; M.D. Baez; C. Alonso ๐Ÿ“‚ Article ๐Ÿ“… 1995 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 660 KB

A continuous flow procedure for the determination of L-asparagine based on its L-asparaginase-catalyzed conversion to L-aspartate and ammonia is proposed. The ammonia formed is measured via the indophenol blue generated in Berthelot's reaction. The enzyme L-asparaginase is covalently bound to an epo