𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Determination of Isocitric Acid in Citrus Juice—A Comparison of HPLC, Enzyme Set and Capillary Isotachophoresis Methods

✍ Scribed by František Kvasnička; Michal Voldřich; Petr Pyš; Ivan Vinš

Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
109 KB
Volume
15
Category
Article
ISSN
0889-1575

No coin nor oath required. For personal study only.

✦ Synopsis

Three different analytical procedures for the determination of dl-isocitric acid content in citrus fruit juices were compared. The dl-isocitric acid is an important marker in multicomponent procedures for the evaluation of authenticity and quality of fruit products. The compared procedures were the new proposed capillary isotachophoresis (CITP) procedure using coupled two capillaries, reverse-phase high-pressure liquid chromatography (HPLC) method and enzymic procedure (Boehringer Mannheim). The optimized CITP electrolyte system was: 6 mm HCl+3.8 mm BisTrisPropan+2mm CaCl 2 +0.05% hydroxypropylmethyl cellulose (leading electrolyte) and 5 mm morpholinethanesulphonic acid+1 mm BisTrisPropan (terminating electrolyte). The best correlation was found between the CITP and enzymic procedure (r=0.956, n=6), the other cross-correlation coefficients were 0.850 and 0.748 for HPLC-enzymic and HPLC-CITP, respectively.

📜 SIMILAR VOLUMES

Determination of (E)-10-hydroxy-2-deceno
Determination of (E)-10-hydroxy-2-decenoic acid content in pure royal jelly: A comparison between a new CZE method and HPLC
✍ Federico Ferioli; Gian Luigi Marcazzan; Maria Fiorenza Caboni 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 366 KB

## Abstract A new CZE method was developed and compared with HPLC for the determination of (__E__)‐10‐hydroxy‐2‐decenoic acid (10‐HDA) in royal jelly (RJ) samples of different geographical origin. The results obtained with the CZE method were highly correlated with those of HPLC (__p__ <0.01). Unde

A modified HPLC method for the determina
A modified HPLC method for the determination of aspartic acid racemization in collagen from human dentin and its comparison with GC
✍ Terezie Benešová; Aleš Honzátko; Alexandr Pilin; Jaroslav Votruba; Miroslav Flie 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 137 KB 👁 1 views

## Abstract The D/L ratio of aspartic acid enantiomers in proteins of low turnover is generally accepted as a reliable procedure for age determination. In our study, twelve samples of eyetooth dentin were analyzed for age determination. The pure insoluble collagen isolated from eyetooth dentin was