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Determination of interleukin 2 activity by a new fluorometric method

✍ Scribed by M. Harvey; B. Pagé; M. Pagé

Publisher
Springer-Verlag
Year
1995
Tongue
English
Weight
311 KB
Volume
9
Category
Article
ISSN
0951-208X

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✦ Synopsis

Interleukin 2 activity is usually determined by a proliferation assay using an IL-Zdependent cell line. Tritiated thymidine incorporation during DNA synthesis is a suitable method for this purpose, but its main drawback is the use of radioactive isotopes. We describe the use of Alamar Blue, a new fluorogenic growth indicator, for the measurement of interleukin 2 activity in microtitration plates. This assay is sensitive and economical. The lower limit of detection is about 400 cells per well with an intra-assay coefficient of variation of about 5 percent.

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