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Determination of inorganic radioiodine in solutions of 125I-labelled proteins

✍ Scribed by Anjrzej Siuda; Barbara Łucka

Publisher
John Wiley and Sons
Year
1981
Tongue
French
Weight
195 KB
Volume
18
Category
Article
ISSN
0022-2135

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✦ Synopsis

Abstract

A rapid paper chromatography procedure using 33% w/v aqueous solution of ammonium sulphate as a solvent has been applied for routine determination of the proportion of 1251 present inorganic iodine in solutions containing 125I‐labelled proteins. R~f~ values for protein, iodide and iodate correspond to 0.0, 0.5, and 0.9, respectively.

The amount of inorganic iodine in the iodination mixture or in the iodinated protein preparation can be determined with good precision in less than 30 minutes.

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