Determination of inert sugars in urine by liquid chromatography with pulsed amperometric detection

✍ Scribed by S.K. Sanghi; W.Th. Kok; G.C.M. Koomen; F.J. Hoek

• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 414 KB
• Volume: 273
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(93)80189-r

✦ Synopsis

bqmd chromatography and pulsed amperometnc detection with a gold workmg electrode have been used for the determmatlon of celloblose and rhamnose m urme samples A conventtonal sugar-separation column was used mth an acldlc mobile phase, and a potassmm hydronde solution was added post-column Under optimal conditions detection hmits of 10 ng were obtained The method could be used for the assessment of the human intestinal permeablhty by the determmatlon of the urinary excretion of mert sugars Lmuts of determmatton m unne were 5 mg 1-l for celloblose and 15 mg 1-l for rhamnose Good agreement was found with the results of alternative methods