Determination of individual cell Michaelis-Menten constants
โ Scribed by Merav Sunray; Naomi Zurgil; Yana Shafran; Mordechai Deutsch
- Publisher
- John Wiley and Sons
- Year
- 2001
- Tongue
- English
- Weight
- 447 KB
- Volume
- 47
- Category
- Article
- ISSN
- 0196-4763
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
An automatic method for the determination of Michaelis-Menten constants of enzymatic reactions in unsegmented flow systems was developed using the variable flow-rate technique, allowing the substrate concentration to be changed during the experiments in order to obtain different reaction rates. The
By initial velocity measurements and two different methods of plotting the experimental data, the K,,, and V,,,, of enzyme action and the first-order rate constant of substrate decomposition can be determined simultaneously under the same conditions. This method permits the determination of K, and V