✦ LIBER ✦

Determination of Michaelis-Menten parameters from initial velocity measurements using unstable substrate

✍ Scribed by Tamás Keleti

Publisher: Elsevier Science
Year: 1986
Tongue: English
Weight: 307 KB
Volume: 152
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(86)90117-x

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✦ Synopsis

By initial velocity measurements and two different methods of plotting the experimental data, the K,,, and V,,,, of enzyme action and the first-order rate constant of substrate decomposition can be determined simultaneously under the same conditions. This method permits the determination of K, and V,,,, even if the presence of the enzyme (or any impurity in the solutions used) influences the rate of substrate decomposition. The theoretical treatment was proved by determining the Michaelis-Menten parameters of D-glyceraldehyde-3-phosphate dehydrogenase and the first-order rate constant of hydrolysis of the unstable substrate, bisphosphoglycerate.

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