Determination of hydroxyproline in tissue
β Scribed by Boyd R. Switzer
- Publisher
- Elsevier Science
- Year
- 1991
- Tongue
- English
- Weight
- 181 KB
- Volume
- 2
- Category
- Article
- ISSN
- 0955-2863
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π SIMILAR VOLUMES
## An ultramicromethod for the determination of hydroxyproline in submicrogram tissue samples is presented. The procedure may be used for the determination of down to 6 ng of hydroxyproline, with a standard deviation of a series of replicates of 0.20 ng. The method has been applied to samples of a
## Abstract Samples of βcollagenβ have been prepared from tendon dissected from shin of beef and found to have a maximum hydroxyproline content of 13.2%, giving a factor of 7.57 for converting hydroxyproline into βcollagenβ. A comparison has been made of the results obtained for the βcollagenβ cont
A simple and practical method for the quantitation of nanogram levels of Chydroxyproline in tissue hydrolysates is described. Optimum conditions were employed to release the hydroxyproline from tissue homogenates by acid hydrolysis. The free hydroxyproline was then oxidized by chloramine T to produc