Connective tissue of meat. III.—Determination of collagen in tendon tissue by the hydroxyproline method
✍ Scribed by L. C. Baker; L. H. Lampitt; K. P. Brown
- Publisher
- John Wiley and Sons
- Year
- 1954
- Tongue
- English
- Weight
- 533 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0022-5142
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✦ Synopsis
Abstract
Samples of ‘collagen’ have been prepared from tendon dissected from shin of beef and found to have a maximum hydroxyproline content of 13.2%, giving a factor of 7.57 for converting hydroxyproline into ‘collagen’. A comparison has been made of the results obtained for the ‘collagen’ content of freeze‐dried tendon preparations (a) by the hydroxyproline method and (b) by the modification of the Lowry, Gilligan & Katersky method previously published.
📜 SIMILAR VOLUMES
## An ultramicromethod for the determination of hydroxyproline in submicrogram tissue samples is presented. The procedure may be used for the determination of down to 6 ng of hydroxyproline, with a standard deviation of a series of replicates of 0.20 ng. The method has been applied to samples of a