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Determination of hydrochlorothiazide in serum by high-pressure liquid chromatography

✍ Scribed by Asbjørg Solberg Christophersen; Knut E. Rasmussen; Bjarne Salvesen

Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
467 KB
Volume
132
Category
Article
ISSN
1873-3778

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✦ Synopsis

A quantitative high-pressure liquid chromatographic method has been developed for the analysis of hydrochlorothiazide in serum in therapeutical concentrations. The method is based on gel filtration of the sera on Sephadex G-15, extraction of the protein-free fraction of the efHuent with ethyl acetate and injection of a methanol solution of the drug extract on a reversed-phase column packed with Spherisorb ODS (particle size, 10 pm). The mobile phase is 15 % methanol in water. The detection limit is 50 ng/ml of serum. Serum samples from patients receiving hydrochlorothiazide have been analysed by the described method at different hours postdose.

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