We have developed a procedure for the accurate measurement of histidine decarboxylase in tissues expressing low levels of enzymatic activity. Briefly, histamine is enzymatically synthesized from [ 3 H]-labeled histidine, followed by purification using high-performance liquid chromatography (HPLC) an
β¦ LIBER β¦
Determination of glutamate decarboxylase by high-performance liquid chromatography
β Scribed by Valeria Rossetti; Anna Lombard
- Book ID
- 103806265
- Publisher
- Elsevier Science
- Year
- 1996
- Weight
- 282 KB
- Volume
- 681
- Category
- Article
- ISSN
- 0378-4347
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## Abstract This work suggests an HPLC method for qualitative and quantitative determination of __N__^Ξ΅^(2βaminoβ2βcarboxyethyl)βLβlysine (LAL). LAL was released from total hydrolysates of various proteins of animal origin and derivatized with dansyl chloride. The performance of two different colum