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Determination of glucose-fructose oxidoreductase activity in whole cells ofZymomonas mobilis

✍ Scribed by G. S. Erzinger; M. M. Silveira; M. Vitolo; R. Jonas

Publisher: Springer
Year: 1996
Tongue: English
Weight: 231 KB
Volume: 12
Category: Article
ISSN: 1573-0972
DOI: 10.1007/bf00327793

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✦ Synopsis

A method for the determination of glucose-fructose oxidoreductase (GFOR) activity in whole cells of Zymomonas mobilis is described. The K m and the theoretical V max for GFOR were 192 g glucose.l(-1) and 17 g gluconic acid.g(-1) cell.h(-1), respectively. The changes in enthalpy (31.1 kJ.mol(-1)), entropy (0.41 kJ.K(-1)), and Gibbs free energy (-97.5 kJ.mol(-1)) related to glucose to gluconic acid conversion were also determined.

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