Improved operational stability of cell-f
✍
Bernd Nidetzky; Monika Fürlinger; Dorothee Gollhofer; Robert K. Scopes; Dietmar
📂
Article
📅
1997
🏛
John Wiley and Sons
🌐
English
⚖ 177 KB
👁 2 views
For the continuous, enzymatic synthesis of sorbitol and gluconic acid by cell-free glucose-fructose oxidoreductase (GFOR) from Zymomonas mobilis, the principal determinants of productivity have been identified. Most important, the rapid inactivation of the soluble enzyme during substrate conversion