Determination of Gemcitabine Triphosphate in Peripheral Blood Mononuclear Cells by Reverse-Phase HPLC
β Scribed by R. Losa; M. I. Sierra; P. Blay; D. Blanco; J. M. Buesa
- Book ID
- 111788019
- Publisher
- Springer
- Year
- 2004
- Tongue
- English
- Weight
- 274 KB
- Volume
- 59
- Category
- Article
- ISSN
- 0009-5893
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## Abstract Gemcitabine triphosphate (dFdCTP) is a highly active metabolite of gemcitabine. It is formed intraβcellularly via the phosphorylation of gemcitabine by deoxycytidine kinase. The monitoring of dFdCTP in human peripheral blood mononuclear cells (PBMCs), in addition to plasma concentration
A simple and sensitive HPLC method is proposed for the determination of glutathione (GSH) in human mononuclear cells, based on the derivatization of the tripeptide with Ellman's reagent. The mobile phase was composed of a mixture of methanol and ammonium formate (10:90 v/v, with a flow rate of 1 mL/