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Determination of furosemide with its acyl glucuronide in human plasma and urine by means of direct gradient high-performance liquid chromatographic analysis with fluorescence detection Preliminary pharmacokinetics and effect of probenecid

✍ Scribed by T.B. Vree; M. van den Biggelaar-Martea; C.P.W.G.M. Verwey-van Wissen

Publisher: Elsevier Science
Year: 1994
Weight: 457 KB
Volume: 655
Category: Article
ISSN: 0378-4347
DOI: 10.1016/0378-4347(94)00093-x

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## Abstract A simple, specific and sensitive HPLC method has been developed for the determination of metoprolol in human plasma and urine. Separation of metoprolol and atenolol (internal standard) was achieved on an Ace C~18~ column (5 μm, 250 mm×4.6 mm id) using fluorescence detection with λ~ex~=2