Determination of fungicide residues in fruits by coupled-column liquid chromatography

✍ Scribed by Tatiana Zamora; Carmen Hidalgo; Francisco J. López; Félix Hernández

• Publisher: John Wiley and Sons
• Year: 2004
• Tongue: English
• Weight: 954 KB
• Volume: 27
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200401760

✦ Synopsis

Abstract

Coupled‐column liquid chromatography with fluorescence detection was applied to the determination of __o‐__phenylphenol and bitertanol residues in orange and banana fruits. After extraction with a mixture of acetone, dichloromethane‐petroleum ether, and ethyl acetate, an extract aliquot of 100 μL was injected directly without any additional clean‐up into the chromatographic system using two reversed phase C~18~ coupled columns. The LC‐LC approach allowed automated sample clean up of the vegetal extracts, leading to a simple and rapid analytical procedure, with limits of quantification between 0.01 and 0.05 mg kg^–1^. Recovery experiments performed on orange and banana samples fortified at different concentrations (0.01–4 mg kg^–1^) gave average recoveries between 70 and 113% with relative standard deviations lower than 15%. The procedure developed was finally applied to orange and banana samples from different geographical locations and the results were confirmed by GC‐MS.