Determination of fluoroquinolone residues in animal tissues by liquid chromatography

✍ Scribed by Andrzej Posyniak; Jan Żmudzki; Stanislaw Semeniuk; Jolanta Niedzielska; Richard Ellis

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 77 KB
• Volume: 13
• Category: Article
• ISSN: 0269-3879
• DOI: 10.1002/(sici)1099-0801(199906)13:4<279::aid-bmc844>3.0.co;2-a

✦ Synopsis

A simple liquid chromatographic (LC) method was developed for the determination of fluoroquinolones (ciprofloxacin, difloxacin, enrofloxacin and sarafloxacin) in animal tissues. Isolation of fluoroquinolones from biological matrices was performed with 5% trichloroacetic acid-acetonitrile (7:3) solution. For clean-up, solid-phase extraction with an SDBI (styrene-divinylobenzene) cartridge was used. LC analyses were performed with analytical column (LiChrospher 100 RP-8 5 mm) and mobile phase (0.025 M O- phosphoric acid-acetonitrile 70:30, v/v) in ion-pair mode. The whole procedure was validated in intra-and inter-assay reproducibility and accuracy determination by simultaneously assaying of muscle, liver and kidney samples supplemented with fluoroquinolones at the level of 30 and 60 ng/g, respectively. The statistical evaluation demonstrates high absolute recovery (b80%) and low coefficient of variation (`10%) for all analysed samples. The detection limits for fluoroquinolones were 5 ng/g in muscle, liver and kidney samples.