✦ LIBER ✦

Determination of free and esterified sterols and of wax esters in oils and fats by coupled liquid chromatography-gas chromatography

✍ Scribed by Konrad Grob; Mauro Lanfranchi; Carlo Mariani

Book ID: 104144560
Publisher: Elsevier Science
Year: 1989
Tongue: English
Weight: 551 KB
Volume: 471
Category: Article
ISSN: 1873-3778
DOI: 10.1016/s0021-9673(00)94187-6

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✦ Synopsis

A liquid chromatographic-gas chromatographic (LC-GC) method is described for the rapid determination of free sterols, esterified sterols and wax esters in oils and fats in a single GC run, circumventing saponification and any off-line preseparation. The free sterols are esterified with pivalic acid in the oil. The diluted oil is injected into the LC system and the fraction containing the above classes of compounds is transferred to the gas chromatograph (carried out by a fully automated LC-GC instrument). The information obtained with this analysis allows a better identification of oils and fats and a better characterization of the treatment of an oil (e.g., distinguishing between cold-pressed and extraction oils and between different refining methods).

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