Determination of fluvoxamine concentration in plasma by reversed-phase liquid chromatography

✍ Scribed by Steven H. Y. Wong; Henry R. Kranzler; Sandra Dellafera; Rosinda Fernandes

• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: English
• Weight: 455 KB
• Volume: 8
• Category: Article
• ISSN: 0269-3879
• DOI: 10.1002/bmc.1130080605

✦ Synopsis

Abstract

Fluvoxamine, a serotonin re‐uptake inhibitor, was quantified in plasma by modifying a previously published procedure for monitoring plasma concentrations of tricyclic antidepressants. Alkalinized plasma samples were extracted with n‐hexane/isoamyl alcohol, followed by back‐extraction with diluted phosphoric acid, The extracts were analysed by reversed‐phase liquid chromatography using a C‐18 column, with phosphate/acetonitrile as the mobile phase. The assay was linear from 10 to 800 μg/L. Precision studies showed within‐run and day‐to‐day coefficients of variation to be 4.5 and 6.8%, respectively. Desipramine interfered with the detection of fluvoxamine. The assay was used to measure a total of 8 plasma samples from 4 alcohol‐dependent patients medicated with fluvoxamine as an adjunct to relapse prevention psychotherapy. In these patients, the plasma concentrations ranged from 54 to 241 μg/L. Dosage of fluvoxamine, duration of treatment, interval between last dosage and blood collection were associated with effects on plasma concentrations that were consistent with the pharmacokinetic profile of fluvoxamine.