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Determination of ester carbonyl 18O16O ratios in phospholipids by gas chromatography-mass spectrometry

โœ Scribed by Patricia C. Schmid; Susan B. Johnson; Harald H.O. Schmid


Book ID
107737137
Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
451 KB
Volume
46
Category
Article
ISSN
0009-3084

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A method for determining the rate of exchange of the carbonyl oxygen in ~-ketoglutarate using gas chromatography-mass spectrometry is described. The method is based on a new procedure for quenching the exchange reaction by rapid oxidative decarboxylation of the LYketoacid to the next lower homologou