We present gas chromatographic/mass spectrometric and gas chromatographic/isotope ratio mass spectrometric assays of the 13C enrichment of plasma urea converted to its dimethylaminomethylene derivative. The limits of sensitivity of the two techniques are 0.2% and 0.02%, respectively. The techniques
Determination of 13C- and 15N-enrichment of glutamine by gas chromatography/mass spectrometry and gas chromatography/combustion/isotope ratio mass spectrometry after N(O,S)-ethoxycarbonyl ethyl ester derivatisation
β Scribed by F. Montigon; J. J. Boza; L. B. Fay
- Publisher
- John Wiley and Sons
- Year
- 2001
- Tongue
- English
- Weight
- 150 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0951-4198
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N(O,S)-Ethoxycarbonyl trifluoroethyl amino acid esters are formed by the reaction of amino acids with ethylchloroformate plus trifluoroethanol plus pyridine. The use of these derivatives for a rapid and sensitive determination of specific enrichment of stable isotopically labeled tracer amino acids
A method for the determination of 15N enrichment and concentration of allantoin and uric acid simultaneously in urine using gas chromatography/mass spectrometry (GC/MS) is described. The urine samples contained acid and its oxidation product allantoin. The uric acid and allantoin were isolated using