Determination of dopamine, norepinephrine, and related trace amines by prechromatographic derivatization with naphthalene-2,3-dicarboxaldehyde

✍ Scribed by Takao Kawasaki; Takeru Higuchi; Kazuhiro Imai; Osborne S. Wong

• Book ID: 102988524
• Publisher: Elsevier Science
• Year: 1989
• Tongue: English
• Weight: 668 KB
• Volume: 180
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(89)90431-4

✦ Synopsis

Dopamine, norepinephrine, octopamine, tyramine, and 3,4-dihydroxybenzylamine react readily with naphthalene-2,3-dicarboxaldehyde in the presence of cyanide ion under mild conditions to give highly fluorescent cyanobenz[f]isoindole (CBI) products. The CBI products exhibit good solution chemical stability. The high-fluorescence quantum efficiency of the CBI fluorophore and the ability to excite these adducts in the visible region (420-450 nm) enhance the sensitivity and selectivity of this derivatization detection technique. The CBI products of catecholamines and"trace" amines are readily separated by reverse-phase HPLC giving detection limits in the 20 to 60 fmol range (SIN = 3). A prechromatographic derivatization HPLC assay for the trace analysis of dopamine and norepinephrine in urine is described.