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Determination of dissociation constants of α-amino acids by ionophoretic technique

✍ Scribed by S. Singh; D. Gupta; M.M. Tewari; P.C. Yadava; S.R. Tripathi; K.L. Yadava

Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
260 KB
Volume
30
Category
Article
ISSN
0013-4686

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✦ Synopsis

A new method involving the use of ionophoretic technique is described for the assessment of dissociation constants of some a-amino acids. viz. glycine, alanine phenylalanine, saline and leucine. The method is based on migration ofa spot of the acids with variation of pH of background electrolytecontaining 0.1 M perchloric acid. A graph of pH against mobility gives information about nature of dissociation and helos in cakuktina them. The calculated values are in fairly good agreement with literature values determined by bther techniques.

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