Determination of dinitrophenylamino acids in structural proteins by chromatography on nylon powder columns : II. The ether-soluble dinitrophenylamino acids

✍ Scribed by H. Beyer; U. Schenk

• Book ID: 104146905
• Publisher: Elsevier Science
• Year: 1969
• Tongue: English
• Weight: 446 KB
• Volume: 39
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)98033-1

✦ Synopsis

SUMhIARY

A procedure is described for the quantitative determination of the ether-soluble dinitropbenyl (DNP)-amino acids in structural protein by column chromatography using nylon powder as adsorbent. The DNP-derivatives of the amino acids: aspartic acid, glutamic acid, serine, tbreonine, glycine, alanine and valine were estimated quantitatively on suck columns by eluting with phosphate buffer, pH S.o, at 60" and at a flow rate of 30 ml per hour. In addition, DNP-cysteic acid, the oxidation product of N,N'-bis-DNP-cystine, was estimated with citrate buffer, p1-I 3.0, at 30" at a flow rate of 30 nil per hour, .