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Determination of deuterium level in biological fluids by isotope ratio mass spectrometry

✍ Scribed by Andrew D. Penman; Iain A. Wright

Publisher
John Wiley and Sons
Year
1987
Tongue
English
Weight
313 KB
Volume
14
Category
Article
ISSN
1076-5174

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✦ Synopsis

Water is produced by vacuum sublimation of biological fluids directly into reaction vessels in preparation for reduction using zinc. Following heating to 450 "C the hydrogen produced is admitted directly to the mass spectrometer. The standard derivation, u,,, of analysis of water samples was 1.08-1.23% while for blood plasma u, = 2.02-2.62%. The method allows the determination of very low levels of deuterium in biological fluids, thus allowing very small doses of deuterium oxide to be used in studies of body water in animals and possibly humans.

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