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Determination of daumone in mouse plasma by HPLC/MS-MS

✍ Scribed by Keumhan Noh; Jong Hee Park; Minkyu Kim; Mankil Jung; Hwa Jeong Lee; Kwang-il Kwon; Wonku Kang; Hunjoo Ha

Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
133 KB
Volume
26
Category
Article
ISSN
0269-3879

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✦ Synopsis

ABSTRACT

Daumone, a pheromone secreted by Caenorhabditis elegans, is an essential regulator of chemosensory processes in development and aging. A quantification method using HPLC/MS‐MS was developed for the determination of daumone in mouse plasma. After simple protein precipitation with acetonitrile including methaqualone (an internal standard), the analytes were chromatographed on a reversed‐phase column and detected by liquid chromatography/tandem mass spectrometry with electrospray ionization. The accuracy and precision of the assay were in accordance with FDA regulations for validation of bioanalytical methods. This method was applied to measure the plasma daumone concentrations following a 5‐week repeated oral administration of daumone in mice. Copyright Β© 2011 John Wiley & Sons, Ltd.

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