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Determination of cortisol in plasma and urine by thin-layer chromatography and fluorescence derivatization with isonicotinic acid hydrazide

✍ Scribed by M. Fenske


Publisher
Springer
Year
1998
Tongue
English
Weight
495 KB
Volume
47
Category
Article
ISSN
0009-5893

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## Abstract Phanquinone (4,7‐phenanthroline‐5,6‐dione) has been investigated as a pre‐column derivatization fluorogenic reagent for liquid chromatographic determination of primary amino acids in biological samples. The derivatization reaction was carried out at 68Β°C both in the presence of aqueous