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Determination of Concentration of Methotrexate Enantiomers in Intracellular and Extracellular Fluids of HepG2Cells by Liquid Chromatography–Tandem Mass Spectrometry

✍ Scribed by Rong Wang, Lifang Guo, Hua Xie, Juanhong Zhang…


Book ID
120897883
Publisher
Springer-Verlag
Year
2013
Tongue
English
Weight
548 KB
Volume
67
Category
Article
ISSN
1085-9195

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A method is described for the evaluation of drug concentrations in plasma and brain from treated rats. The analyte is recovered from plasma or brain homogenate by liquid-liquid extraction and subsequently analyzed by liquid chromatography/tandem mass spectrometry (LC/MS/MS). A simple experimental pr