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Determination of clindamycin in animal plasma by high-performance liquid chromatography combined with electrospray ionization mass spectrometry

✍ Scribed by Marc Cherlet; Siska Croubels; Patrick De Backer

Publisher
John Wiley and Sons
Year
2002
Tongue
English
Weight
117 KB
Volume
37
Category
Article
ISSN
1076-5174

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✦ Synopsis

Abstract

A method for the quantification of clindamycin in animal plasma using high‐performance liquid chromatography combined with electrospray ionization mass spectrometry (LC/ESI‐MS/MS) is presented. Lincomycin is used as the internal standard. The sample preparation includes a simple deproteinization step with trichloroacetic acid. Chromatographic separation is achieved on an RP‐18 Hypersil column using gradient elution with 0.01 M ammonium acetate and acetonitrile as mobile phase. Good linearity was observed in the range 0–10 µg ml^−1^. The limit of quantification of the method is 50 ng ml^−1^ and the limit of detection is 1.3 ng ml^−1^. The method was shown out to be of use for pharmacokinetic studies of clindamycin formulations in dogs. Copyright © 2002 John Wiley & Sons, Ltd.

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