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Determination of chlorophylls by reversed-phase high-performance liquid chromatography with isocratic elution and the column-switching technique

โœ Scribed by Koichi Saitoh; Izumi Awaka; Nobuo Suzuki

Book ID
107785264
Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
326 KB
Volume
693
Category
Article
ISSN
1873-3778

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Free amino acids (FAA) were separated by reversed-phase ion-pair liquid chromatography using a column-switching technique with isocratic elution. The eluted FAA were monitored by fluorescence detection using o-phthalaldehyde reagent. A 5-,um phenyl-bonded silica gel column (5 cm x 4.6 mm I.D.) was u