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Determination of cells' water membrane permeability: Unexpected high osmotic permeability of Saccharomyces cerevisiae

✍ Scribed by I. Martínez de Marañón; P. Gervais; P. Molin

Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
110 KB
Volume
56
Category
Article
ISSN
0006-3592

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✦ Synopsis

Water permeability (Lp), calculated from the volume variations of cells subjected to an osmotic shock, is classically used to characterize cell membrane properties. In this work, we have shown the importance of the kind of mixing reactor used to measure the Lp parameter. A mathematical model including the mixing time constant has been proposed allowing an accurate Lp estimation even though the mixing time constant is higher than the cell time constant obtained in response to a perfect shock. The estimated Lp values of human leukemia K562 cells were found to be the same whatever the mixing time constant. The Lp value of Saccharomyces cerevisiae could not be exactly estimated. However, S. cerevisiae has unexpectedly high water permeability, im- plying that this yeast may contain water channels in the membrane.

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